Description

Kit Test ELISA Staphylococcus Enterotoxin trong Sữa

 Introduction

1. Enterotoxin (SE) is a pathogenic substance that causes Staphylococcus aureus food poisoning. According to its antigenicity, it can be divided into 8 serotypes A-E and G-I, of which SEA is the most common. Enterotoxins in food are not easily inactivated during processing and can cause symptoms such as vomiting and diarrhea. In severe cases, they can cause multiple organ dysfunction and be life-threatening.
2. This kit is a new generation of Staphylococcus aureus total enterotoxin (SET) detection product using ELISA , which can minimize operating errors and work intensity.

Principle

This kit adopts double antibody sandwich ELISA method. Pre-coat the total amount of Staphylococcus aureus enterotoxin antibody on the microplate. Both the total amount of pre-coated enterotoxin antibody and detection antibody bind to the enterotoxin in the sample at the same time. And then the complex combines with the HRP-conjugate to form an antibody- enterotoxin- antibody- enzyme- HRP-conjugate, which can be colored by TMB substrate. The absorbance of the sample is positively correlated with the content of Staphylococcus aureus enterotoxin.

 Parameters

Sensitivity: 0.25-1ppb

Limit of Detection (Coefficient of Variation of the kit is less than 10%):

 Materials Required but Not Provided

1. 
   

   Devices:

—- Microwell plate Reader (450nm/630nm)

—- Vortex

—- Incubation container

—- Refrigerated centrifuge

—- Balance (sensibility: 0.01g)

—- Centrifuge tube: 2mL, 5mL, 7mL, 50mL

—-Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)  Multi-channel pipet (30µL -300µL)

2. 
   

   Reagent:

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

—- Sodium hydroxide (1mol/L)

Kit components