Kit Test ELISA Staphylococcus Enterotoxin trong Sữa

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Kit Test ELISA Staphylococcus Enterotoxin

It can qualitatively detect the total content of Staphylococcus aureus enterotoxin in raw milk, finished milk, fermented milk, milk beverages, whey powder and milk powder samples.

  • LOD: 0.025-1 ppb
  • 96T
  • Sample: Raw milk, Milk products
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Mô tả

Kit Test ELISA Staphylococcus Enterotoxin trong Sữa

Manufacturer Meizheng
Order No. LR50501
Analyte Staphylococcus aureus enterotoxin
Sample Type Raw milk, finished milk, fermented milk, milk beverages, whey powder and milk powder samples.
Methodology Double antibody sandwich ELISA method
Packaging 96 Tests
Storage T  The storage temperature for the kits is 2-8℃.
Shelf life The validity for this kit is 12 months
Lead time Shipped within 5-12 working days.

 Introduction

  1. Enterotoxin (SE) is a pathogenic substance that causes Staphylococcus aureus food poisoning. According to its antigenicity, it can be divided into 8 serotypes A-E and G-I, of which SEA is the most common. Enterotoxins in food are not easily inactivated during processing and can cause symptoms such as vomiting and diarrhea. In severe cases, they can cause multiple organ dysfunction and be life-threatening.
  2. This kit is a new generation of Staphylococcus aureus total enterotoxin (SET) detection product using ELISA , which can minimize operating errors and work intensity.

Principle

This kit adopts double antibody sandwich ELISA method. Pre-coat the total amount of Staphylococcus aureus enterotoxin antibody on the microplate. Both the total amount of pre-coated enterotoxin antibody and detection antibody bind to the enterotoxin in the sample at the same time. And then the complex combines with the HRP-conjugate to form an antibody- enterotoxin- antibody- enzyme- HRP-conjugate, which can be colored by TMB substrate. The absorbance of the sample is positively correlated with the content of Staphylococcus aureus enterotoxin.

 Parameters

Sensitivity: 0.25-1ppb

Limit of Detection (Coefficient of Variation of the kit is less than 10%):

Sample LOD (ppb)
SEA SEB SEC SED SEE
Fresh milk, finished milk, whey powder, milk powder 0.5 0.25 0.25 0.25 1
Fermented milk 3 1.5 1.5 1.5 6
milk beverages 1 0.5 0.5 0.5 2

 Materials Required but Not Provided


  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Vortex

—- Incubation container

—- Refrigerated centrifuge

—- Balance (sensibility: 0.01g)

—- Centrifuge tube: 2mL, 5mL, 7mL, 50mL

—-Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)  Multi-channel pipet (30µL -300µL)


  1. Reagent:

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

—- Sodium hydroxide (1mol/L)

Kit components

Component 96 wells
Microwell plate 12 test strips of 8 wells each
SET antibody solution 12ml
SET Enzyme conjugate 12ml
Substrate A 6ml
Substrate B 6ml
Negative control 3ml
Positive control 3ml
Concentrated wash buffer (10×) 40ml *2
Insert 1
Cover sheet 2

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