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BỘ KIT KIỂM TRA DƯ LƯỢNG KHÁNG SINH TRONG SỮA (DASH)
Dairy Antibiotics Spore High-throughput (DASH) Test Kit
| Analyte | Antibiotic residues |
| Sample Type | Raw milk (including cattle, sheep, and goats). |
| Methodology | Microbial inhibition |
| Packaging | 96 Tests |
| Storage | 4-8℃ |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
Principle of Test
This kit adopts the principle of microbial inhibition. The microwell plate is filled with a medium containing Geobacillus stearothermophilus spores and the pH indicator. When cultured at 65℃, the spores germinate, the bacteria grow, and then metabolize to produce acid. The pH value decreases and the indicator changes from the original purple to yellow. If the antibiotics exist in the added milk and dairy products, the antibiotics will inhibit bacterial growth, thus no acid is produced, the indicator does not change the color, and the original purple is maintained; if the antibiotics do not exist in the added milk and dairy products or the concentration of antibiotics is below the detection limit, the spores germinate, metabolize and product acid, and the medium turns yellow.
Detection Limit
| Antibiotic | Detection Limit (μg/L) |
| Beta-lactams | |
| Penicillin G | 2 |
| Ampicillin | 2-3 |
| Amoxicillin | 2-3 |
| Nafcillin | 5 |
| Cloxacillin | 10-15 |
| Oxacillin | 6 |
| Dicloxacillin | 10-15 |
| Cefacetrile | 20 |
| Cefalexin | 45 |
| Cefalonium | 5-6 |
| Cefoperazone | 30 |
| Ceftiofur | 50 |
| Cefquinome | 75 |
| Cefapirin | 2.5-5 |
| Cefazolin | 10-15 |
| Tetracyclines | |
| Chlortetracycline | 200 |
| Doxycycline | 75-100 |
| Oxytetracycline | 100-150 |
| Tetracycline | 100-150 |
| Aminoglycosides | |
| Gentamicin | 100 |
| Kanamycin | 2500 |
| Neomycin | 200-300 |
| Spectinomycin | >300 |
| Streptomycin | 1100-1500 |
| Dihydrostreptomycin | 700-1000 |
| Sulfonamides | |
| Sulfamethazine | 50-100 |
| Sulfadimethoxine | 25-50 |
| Sulfamerazine | 100 |
| Sulfathiazole | 40 |
| Sulfadiazine | 50 |
| Sulfadoxine | 80-120 |
| Sulfamethoxazole | ＜25 |
| Sulfamethoxpyridazine | 50-100 |
| Sulfisoxazole | 50 |
| Sulfachloropyridazine | 25-50 |
| Sulfaphenazole | 25-50 |
| Sulfamethoxydiazine | 50-100 |
| Sulfabenzamide | 100 |
| Sulfaclozine | 100 |
| Sulfapyrazole | 50 |
| Sulfamethoxypyrazine | 25-50 |
| Sulfisomidine | 50-100 |
| Sulfamonomethoxine | 50 |
| Sulfapyridine | 100 |
| Sulfaquinoxaline | 50 |
| Sulfamethazole | 100 |
| Sulfamethizole | 50-100 |
| Macrolides | |
| Erythromycin | 100-150 |
| Spiramycin | 400-800 |
| Tilmcosin | 100 |
| Tylosin | 40-80 |
| Quinolones | |
| Ciprofloxacin | 1000-1500 |
| Danofloxacin | 1000-2000 |
| Marbofloxacin | ＞1000 |
| Various | |
| Chloramphenicol | 2500 |
| Rifaximin | 60-100 |
| Pirlimycin | 200 |
| Thiamphenicol | ＞1000 |
| Trimethoprim | 100 |
| Lincomycin | 300-500 |
Reagents and Materials Supplied
| Detachable microwell plate | 3 pieces
(96 independent tests each piece) |
| Cover film (Aluminum foil) | 3 |
| User manual | 1 |
| Colorimetric card | 1 |
Materials required but not supplied
—- Stable 65°C heating equipment (water bath or dry heater)
—- Single channel micropipette (2.5-100µL)
—- Negative control: milk without antibiotics
—- Positive control: milk containing 10mg/L Penicillin G or 10mg/L Ampicillin
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